Journal: Autophagy
Article Title: LAMP2-FLOT2 interaction enhances autophagosome-lysosome fusion to protect the septic heart in response to ILC2
doi: 10.1080/15548627.2025.2469207
Figure Lengend Snippet: ILC2 is the major IL4-producing cell subset in septic heart. (A, B) Representative flow cytometry plots (A) and bar graph (B) of percentages of IL4-, IL9-, IL13-, and IL5-positive ILC2 in the hearts at the indicated time points after CLP surgery. (C, F) Representative flow cytometry plots (C) and bar graph (F) of percentages of IL4-positive T cells in the hearts after CLP or sham surgery. (D, E) the expression levels of IL4 on CD3-positive T cells (D) and CD4-positive T cells (E) in the hearts after CLP or sham surgery. (G, H) ILC2 were depleted with anti-THY1 antibody and were analyzed by flow cytometry. (I, J) the expression levels and mean fluorescence intensity (MFI) of IL4 on ILC2 in the hearts. The data are expressed as mean ± SEM ( n = 4–5/group). In B, two-way ANOVA with Turkey’s multiple comparisons tests; in F, unpaired Student’s t-test; in H and J, one-way ANOVA. ns, no significance; * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.
Article Snippet: Plasma levels of IL4 (MULTI SCIENCES, EK204), IL5 (MULTI SCIENCES, EK205), IL9 (MULTI SCIENCES, EK209), and IL13 (MULTI SCIENCES, EK213) protein levels were detected using mouse ELISA kits.
Techniques: Flow Cytometry, Expressing, Fluorescence